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GeneFix® Streptavidin (GFS) is
a revolutionary advancement in the application of solid phase techniques to molecular
biology applications. GeneFix® tubes are produced from standard 200 µl
test tubes (strips). The inner wall of these tubes is pre-treated to facilitate the
immobilization of nucleic acids of your interest. The nucleic acids can be fixed to the
inner wall of GeneFix® tubes at the biotin moiety of nucleic acid derivatives. This tool provides a rapid, simple, powerful and cost effective alternative
for the isolation, separation, enzymatic synthesis and detection of nucleic acids. 
Properties
- GFS tubes are available as a kit consisting of 12 strips of eight 200 µl tubes along with a standard 8 x 12 microtiter plate format tray. The GFS tubes can be used either individually or simultaneously in an array format.
- GFS tubes can be stored at +4 oC for several months without degradation of the binding capacity or any other properties. (Freezing is not recommended.)
- GFS tubes are supplied in three configurations corresponding to the amount of binding area within the tubes. The smallest configuration is 50 µl, the medium is 100 µl and the largest configuration is 200 µl.
- The binding capacity of GFS tubes is fragment length dependent. Steric hindrance causes reduced binding capacity increasing the molecular weight of fragments. Binding capacity is measured with the Bi-(dT)25 standard. The binding capacity of GFS tubes is more than 10, 20 and 40 pmol corresponding to the effective 50, 100 and 200 µl covered surfaces.
- Non-specific binding of GFS tubes is less than 1% both for nucleotides and oligonucleotides.
Recommendations
- The biotin-streptavidin complex can withstand various "harsh" conditions without dissociation of the biotin-streptavidin link (e.g. 8 M urea or 1 M guanidine-HCl). However, in some cases the target material can release from the surface of the tube. You should avoid biotin-streptavidin linkage destroying circumstances e.g., high temperature (>80 oC), dimethylformamide or dimethylsulfoxyde at elevated temperature, etc., otherwise, you can release the immobilized, biotinylated strand this way.
- To reduce steric hindrance all biotin reagents should contain a spacer arm, at least 6 A in length.
- Pipette tips are made of hard polypropylene. To avoid loss of the immobilized material be careful not to scratch the wall of the tube.
- Free biotin will reduce the binding capacity of GFS tubes. Removing of any unincorporated biotin from samples is recommended.
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